Xylella fastidiosa Plasmid-Encoded PemK Toxin Is an Endoribonuclease
نویسندگان
چکیده
منابع مشابه
ANALYSIS OF XYLELLA FASTIDIOSA TRANSPOSON MUTANTS AND DEVELOPMENT OF AN EN PLANTA XYLELLA FASTIDIOSA PLASMID VECTOR Project Leader: Cooperator:
We have developed an autonomously replicating Xylella fastidiosa (Xf)/E. coli plasmid that efficiently transforms Xf; unfortunately this plasmid was not stably maintained in Xf cells en planta or without antibiotic selection in vitro. Another plasmid, containing an Xf native plasmid, a Kan cassette and cloned in pUC18 was also constructed and shown to be unstable without antibiotic selection. 1...
متن کاملFunctional characterization of replication and stability factors of an incompatibility group P-1 plasmid from Xylella fastidiosa.
Xylella fastidiosa strain riv11 harbors a 25-kbp plasmid (pXF-RIV11) belonging to the IncP-1 incompatibility group. Replication and stability factors of pXF-RIV11 were identified and used to construct plasmids able to replicate in X. fastidiosa and Escherichia coli. Replication in X. fastidiosa required a 1.4-kbp region from pXF-RIV11 containing a replication initiation gene (trfA) and the adja...
متن کاملA Conjugative 38 kB Plasmid Is Present in Multiple Subspecies of Xylella fastidiosa
A ≈ 38kB plasmid (pXF-RIV5) was present in the Riv5 strain of Xylella fastidiosa subsp. multiplex isolated from ornamental plum in southern California. The complete nucleotide sequence of pXF-RIV5 is almost identical to that of pXFAS01 from X. fastidiosa subsp. fastidiosa strain M23; the two plasmids vary at only 6 nucleotide positions. BLAST searches and phylogenetic analyses indicate pXF-RIV5...
متن کاملAnalysis of Xylella Fastidiosa Transposon Mutants and Development of Plasmid Transformation Vectors
We screened over 1,000 random Tn5 Xylella fastidiosa (Xf) mutants in Chardonnay grapevines growing in the greenhouse in 2003. Approximately 10 of the mutants exhibited a hypervirulent phenotype, i.e. vines inoculated with these mutants developed symptoms sooner and died sooner than vines inoculated with the wild type Xf parental strain. The identity of the Tn5 insertion sites in these mutants w...
متن کاملMapping analysis of the Xylella fastidiosa genome.
A cosmid library was made of the 2.7 Mb genome of the Gram-negative plant pathogenic bacterium Xylella fastidiosa and analysed by hybridisation mapping. Clones taken from the library as well as genomic restriction fragments of rarely cutting enzymes were used as probes. The latter served as a backbone for ordering the initial map contigs and thus facilitated gap closure. Also, the co-linearity ...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2012
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto-05-11-0150